Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-aged and Aged Organophosphoryl Conjugates of AChE
Abstract
The objective of this project is to detect and characterize, by nmr and fluorescence spectroscopy, differences in the conformation of non-aged and aged organophosphoryl (OP) conjugates of acetylcholinesterase (AChE) and alpha- chymotrypsin (a-Cht). Two series of novel fluorescent OP inhibitors were prepared, containing either pyrenebutyl or dansylaminoethyl moieties; these inhibitors were used to obtain homologous pairs of non-aged phosphorylated conjugates of alpha-Cht. The techniques employed to characterize these conjugates, and to detect differences between homologous aged and non-aged conjugates, included kinetic measurements of inhibition, reactivation, and aging, 31P-nmr spectroscopy, and circu-larly polarized luminnescence (CPL). The main findings and principal conclusions are summarized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1985
- Accession Number
- ADA202381
Entities
People
- I. Silman
- Y. Ashani
Organizations
- Israel Institute for Biological Research