Human Immune Response to Dengue Infections
Abstract
We have begun to analyze human T cell responses to dengue antigens in vitro to elucidate the possible role of T lymphocytes in the pathogenesis of dengue hemorrhagic fever and dengue shock syndrome. Dengue antigens induce proliferative responses of peripheral blood mononuclear cells (PBMC) from dengue antibody-positive donors, but do not induce specific proliferative responses of PBMC from dengue antibody-negative donors. Interferon gamma is detected in the culture fluids of dengue-immune PBMC stimulated with dengue antigens. Dengue- specific T cell clones were established using lymphocytes from a donor who was known to be infected with dengue 3 virus. They have CD3+, CD4+ and CD8- phenotypes, and produce IFN(gamma) in response to dengue Ag. One clone examined to date has cytotoxic activity to dengue-infected autologous cells. The effect of IFN (gamma) on dengue virus infection of human monocytic cells was studied. These results indicate that PBMC from dengue-immune donors contain CD4+ T cells which proliferate and produce IFN (gamma) after stimulation with dengue antigens, and suggest that the IFN (gamma) which is produced by these stimulated dengue-specific T cells may contribute to the pathogenesis of dengue hemorrhagic fever and dengue shock syndrome by increasing the number of dengue virus- infected monocytes in the presence of cross-reactive anti-dengue antibodies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 31, 1988
- Accession Number
- ADA202453
Entities
People
- Francis A. Ennis
Organizations
- University of Massachusetts