The Role of Newly Discovered Exotoxin (S Toxin) in Pseudomonas aeruginosa Infections
Abstract
The long term goal is to determine the role of exotoxin S in humans infected with Pseudomonas aeruginosa and develop vaccines to reduce the morbidity and mortality associated with these infections. During the period of the project covered in this annual report (August 1, 1981 - December 31, 1982), we have: 1) Purified two distinct forms of protein S, partially characterized these proteins and continued to characterize the lipid vesicles (found in culture supernatant which contain the S proteins; 2) prepared specific antisera in rabbits against the two (separate forms of S protein and used these antisera to immunologically characterize the two S proteins and develop a better (more sensitive and specific) assay for S(+) colonies (for mutant and cloning studies) ; 3) Continued to investigate the role of S in chronic pulmonary infections; 4) Continued our genetic studies on S. Ten mutants were isolated, Characterized and found unsuitable for virulence studies. Alternative approaches were developed on which our future studies are based. (KR/KT)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1983
- Accession Number
- ADA202868
Entities
People
- Barbara H. Iglewski
- Donald E. Woods
- Larry Hanne
- Thalia Nicas
Organizations
- Oregon Health & Science University