Isolation of Genomic Clone for human Cholinesterase
Abstract
The cDNA for human cholinesterase (EC 3.1.1.8) was isolated from a human basal ganglia library. The clone has a total length of 2426 base pairs (bp), of which 1722 bp code for the mature protein, 212 bp are on the 5 side of the coding sequence, and 492 bp are in the 3' untranslated region. The signal peptide contains 28 amino acids. The amino acid sequence deduced from the cDNA exactly matched the 574 amino acid sequence of the cholinesterase found in human plasma. Therefore, our cDNA represents cholinesterase (EC 3.1.1.8) rather than acetylcholinesterase (EC 3.1.1.7). It was concluded that the amino acid sequence of cholinesterase from two different human tissues, brain and serum, was identical. Hybridization of genomic DNA blots suggested that a single gene or very few genes, coded for cholinesterase. A genomic clone was isolated using CDNA as a probe. The genomic clone contained exons 1 and 2. There appear to be a minimum of four exons in the cholinesterase gene, and we are searching for other genomic clones containing exons 3 and 4.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1987
- Accession Number
- ADA202965
Entities
People
- Oksana Lockridge
Organizations
- University of Michigan