Cloning and Expression of Genes for Dengue Virus Type-2 Encoded-Antigens for Rapid Diagnosis and Vaccine Development

Abstract

Using oligodeoxynucleotide primers, we cloned the region of DEN-2 RNA encoding the structural proteins C, prM (M), and E glycoprotein. The cDNA clones were completely sequenced. Using a synthetic primer complementary to the 3'-end of DEN-2 RNA, additional cDNA clones, mapping 3' to the previously characterized clones, A4 and B2 were isolated. Genome 'walking' using these cDNA clones as probes, pPM-F12, pPM-A10, pPM53 were isolated, all mapping toward the 3'-end of the genome. These cDNA clones were sequenced. To complete the cDNA cloning of DEN-2 genome, a novel approach was used which involved the polymerase chain reaction. Using this approach, the sequences between nucleotide 9950 and the 3'- terminus were amplified in a Taq polymerase-catalyzed chain reaction. The amplified DNA was cloned and sequenced. Keywords: Dengue, Vaccines, Diagnosis medicine.

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Document Details

Document Type
Technical Report
Publication Date
Oct 31, 1988
Accession Number
ADA203018

Entities

People

  • Radha K. Padmanabhan

Organizations

  • University of Kansas Medical Center

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Artificial Intelligence
  • Chemical Reactions
  • Chemistry
  • Classification
  • Coding
  • Dengue
  • Dna Sequence Analysis
  • Medical Personnel
  • Molecular Biology
  • Polymerase Chain Reaction
  • Polyproteins
  • Proteins
  • Sequence Analysis
  • Vaccines
  • Virion
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology