The Role of Newly Discovered Exotoxin (S Toxin) in Pseudomonas aeruginosa Infections
Abstract
Our long term goal is to determine the role of exotoxin S in humans infected with Pseudomonas aeruginosa and develop vaccines to reduce the morbidity and mortality associated with these infections. We have: 1) Obtained direct evidence that exotoxin S is produced in humans infected with an S+ strain of P. aeruginosa, 2) Begun to investigate the role of S in chronic pulmonary disease due to P aeruginosa using a rat model, 3) Developed a Welek assay and a filter blot RIA for identifying mutants of P. aeruginosa altered in S production, 4) Identified a proenzyme form of S which is 4,000 daltons larger than the enzymatically active form of S, 5) Extended and completed a survey of clinical isolates to determine what percentage produces exotoxin S, 6) Continued to collaborate with Drs. C. Alving, A. Cross and J. Sadoff (Walter Reed Army Institute of Research) on Pseudomonas research and purified and supplied them with purified diphtheria toxin and Pseudomonas toxin A.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1981
- Accession Number
- ADA203064
Entities
People
- Barbara H. Iglewski
- Donald E. Woods
- Michael R. Thompson
Organizations
- Oregon Health & Science University