Electrophysiological and Electrochemical Methods Development for the Detection of Biologically Active Chemical Agents
Abstract
The patch microprobe system is an inquisitively sensitive electrophysiological methodology for measuring the transduction of electrical signals in biomembranes and/or trans-membrane ionic conductances. Progress has been made in various phases of the patch microprobe project: the creation of planar phospholipid bilayers, reconstitution of extracts from mammalian tissue, development of recording chambers, and formation of an artificial bio-membrane patch. The next phase of this project will be to transfer the active receptor complex to an artificial membrane. This system will be useful in testing biological receptors for their ability to detect and to discriminate selected threat agents in a bio-sensor scheme. Keywords: Cholinergic drugs; Acetylcholinesterase; Muscarinic receptors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1988
- Accession Number
- ADA203099
Entities
People
- George G. Bierkamper
- James J. Valdes
Organizations
- University of Nevada, Reno School of Medicine