Strategy for Isolation of Gene Activation Factors
Abstract
How genes one another has been a central problem in biology. One mechanism is that certain genes produce trans-activating factors, which stimulate others, but the former have been difficult to isolate since they may be distant from the latter. This report describes a possible solution to this problem. Recombinant DNA technology can be used to construct a vector composed of gene regulatory (e.g. promoter) sequences fused inframe to a reporter gene, plus a selectable marker. When this vector is placed into permissive host cells, transfection of additional DNAs (syngeneic with the source of the regulatory sequences) may serve to activate the reporter gene. These new DNAs, which encode 'trans-activating factors,' then can be rescued with species-specific or linked probes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1988
- Accession Number
- ADA203112
Entities
People
- Henry Chang
Organizations
- Naval Medical Research Center