Cloning and Structure of Different Types of Spider Silk

Abstract

Amino acid sequence from several spider silk proteins have been determined. These include: Nephila dragline (GYGPG, GQGAG, GAGQG, GYGGLG) and cocoon (SAFQ) and Araneus dragline (GPYGPGQQGP) and cocoon (FLGG, SVGLV-LI-A-Y- A-L). Over 18 positive clones have been identified from a Nephila silk gland library using an 18 mer probe based on the dragline protein sequence. These have been plague purified and sequencing has started. Libraries for the other silks are being constructed. Using FTIR (with microscope focus) changes in the IR region have been detected when the silk is stretched. Efforts are now underway to determine what structural features these changes correspond to. Keywords: Protein, Silk, Elasticity.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1988
Accession Number
ADA203137

Entities

People

  • Randolph V Lewis

Organizations

  • University of Wyoming

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Chemical Compounds
  • Classification
  • Contracts
  • Elastic Properties
  • Genetic Structures
  • Military Research
  • Peptides
  • Rocky Mountains
  • Security
  • Sequences
  • Students
  • Universities
  • X Rays
  • X-Ray Diffraction

Readers

  • Allergy and Immunology.
  • Immunology
  • Naval Engineering and Maritime Security