Evaluation of Pharmacologic Agents to Suppress Intraocular Cellular Proliferation Following Trauma
Abstract
As limits of mechanical vitreoretinal surgical techniques are being reached, it has become clear that the main cause of lost eyes from trauma and proliferative vitreoretinopathy is due to intraocular cellular proliferation. Several agents have been suggested to control intraocular cellular proliferation. This study was designed to test these agents (5-FU, methotrexate, prostaglandin P G E 1, dexamethasone, trimacinolone, indomethacin, colchicine, d-penicillamine) against control eyes as well as to test the toxicity at timing of injection. In a rabbit model of tractional retinal detachment using retinal pigment epithelial cells as an injected cell, bolus pharmacologic agents were tested under matched conditions both early after cell injection and late after cells were allowed to divide. Clinical detachment, light microscopic evaluation of cellular proliferation, and electron microscopic evaluation of retina for toxicity and electrophysiologic testing were all used to evaluate each drug. We found 5-FU and P G E 1 to show the greatest promise for suppression of clinical detachment and lower retinal toxicity in the retinal rabbit model for early and late injection of cells respectively. These drugs attack these cells at various points in the synchronized cell life cycle in the animal model. Due to the lack of a synchronized cell cycle in the tractional detachment of trauma, it may be advisable to consider a bolus of several drugs affecting both cell division as well as cell contractility.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1986
- Accession Number
- ADA203198
Entities
People
- Gary L. Blanchard
- Hedwig A. Murphy
- Michael T. Trese
Organizations
- Michigan State University