Effects of Hyperbaric Pressure on Tissue Phospholipid Metabolism
Abstract
We have been pursuing the characterization of subcellular and tissue culture preparations prior to initiating hyperbaric pressure studies. We have been successful in applying free-flow electrophoresis to the further purification of synaptosomes and lysosomes, after initial purification by centrifugation. These are being studied for phospholipid composition and enzymatic activity, including the phospholipases. We have succeeded in culturing and establishing a culture line of astrocytes in our tissue culture facility. Immunocytochemical studies are under way for morphological characterization of these cells. The endothelial cell culture system has also been established and additional morphological endpoints, including antibody techniques, are being developed prior to extending this work to the hyperbaric pressure studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 27, 1987
- Accession Number
- ADA203208
Entities
People
- William B. Weglicki
Organizations
- George Washington University