Effects of Toxins on Arachidonic Acid Metabolism in Cultured Rat Pulmonary Alveolar Macrophages

Abstract

The action of a trichothecene (T-2), microcystin-LR and saxitoxin on arachidonic acid metabolism in cultured rat alveolar macrophages was studied. Pulmonary macrophages exposed to 1 micromole T-2 were stimulated to synthesize and increase in release thromboxane B2 (TxB2) by 56% and 6-KetoF 1 alpha (73%). Microcystin-LR induced a significant increase in release of prostaglandins F 2 alpha, (40%), PGE2 (75%) and TxB2(69%, as compared to controls. Saxitoxin increased TxB2 release by 37%. Arachidonic acid release was stimulated by all three toxins tested. The release of arachidonic acid and its metabolites by alveolar macrophages exposed to T-2 toxin was partially blocked by Fluocinolone (imicromate). These results suggest that macrophages synthesize and release inflammatory mediators in response to toxin exposure, and fluocinolone may protect against T-2 toxicosis.

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Document Details

Document Type
Technical Report
Publication Date
Dec 28, 1988
Accession Number
ADA203314

Entities

People

  • Donald A. Creasia
  • Harry B. Hines
  • Syed M. Naseem

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Analysis Of Variance
  • Biomedical Research
  • Cells
  • Chloroform
  • Classification
  • Dermatologic Agents
  • Fungi
  • Lethal Dosage
  • Macrophages
  • Materials
  • Measurement
  • Metabolism
  • Metabolites
  • Production
  • Proteins
  • Security

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Mycotoxin ecology in Amazonian ecosystems.