Influence of Neuroendocrine Mediators on Phagocyte Function
Abstract
The objective of this research program was to explore the effects of certain neuroendocrine mediators and macrophage function. Treatment with met- enkephalin led to a flattened cell morphology with many linear and punctate microfilaments in the cytoplasm. These physical changes in cell structure were accompanied by dose-dependent increases in phagocytosis, spreading and adherence whereas cytolysis was not affected. In contrast, epinephrine treatment led to a spherical cell morphology and a dense cortical band of F-actin. Macrophage phagocytosis and spreading were decreased in a dose-dependent fashion whereas adherence and cytolysis were unaffected. Propranolol reversed the effects of epinephrine. The effects of epinephrine were mimicked by cAMP analogs and forskolin. Combined experiments using optimal met-enkephalin doses and various doses of epinephrine gave results identical to that of epinephrine alone. These receptor signaling pathways may interact via protein kinases. Protein kinase C activators and inhibitors enhance and depress macrophage spreading, respectively. Cyclic AMP antagonizes the action of protein kinase C activators. Protein kinase systems may transduce multiple neuroendocrine signals into physiological responses via effects on granules and the cytoskeleton. Keywords: Stress physiology, Opioid, Adrenergic nerves, Neurochemistry, Immunology.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1988
- Accession Number
- ADA203364
Entities
People
- Howard R. Petty
Organizations
- Wayne State University