Genetics in Marine Methane-Oxidizing Bacteria

Abstract

The purpose of this project is to characterize the regulation of genes involved in methanol oxidation in the marine methanotroph, Methylomonas sp. A4. In the first year of this project, we have isolated and characterized methanol oxidation (Mox) genes, including moxF, encoding the 60kD subunit of the methanol dehydrogenase (MeDH), moxl, encoding the 10kD subunit of the MeDH and moxA3, encoding a function involved in apoprotein-cofactor assembly of the MeDH. We have also identified a putative moxG region, encoding the MeDH-specific cytochrome c. In an effort to develop useful mutagenesis systems in Methylomonas A4, we have tested transposon delivery systems, and have obtained low level transposition with one vector, PSUP201: TN5-21. Keywords: Genetics.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1989
Accession Number
ADA203790

Entities

People

  • Mary E. Lidstrom

Organizations

  • California Institute of Technology

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Alcohols
  • Alkenes
  • Amino Acids
  • Bacteria
  • Classification
  • Coding
  • Contracts
  • Cytochromes
  • Genetics
  • Identification
  • Methanols
  • Military Research
  • Notation
  • Oxidation
  • Resistance
  • Symbols

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Organic Chemistry

Technology Areas

  • Biotechnology