Pathophysiology and Toxicokinetic Studies of Blue-Green Algae Intoxication in the Swine Model
Abstract
The need to conserve toxin and the goal of creating a model for naturally occurring toxicosis caused us to evaluate the oral administration of microcystin-A (MCYST-A) to rodents. But in practice, purified MCYST-A has demonstrated low acute toxicity when administered to laboratory animals by the oral route. Development of an in vivo isolated intestinal loop preparation in the rat has provided a model system for investigating the enteric absorption site of MCYST-A on toxicity. Using this system, MCYST-A toxicity was observed to be greater from the ileum than the jejunum as measured by increased liver weight. Also, cholestyramine was evaluated as a therapeutic agent and found to decrease the toxicity of MCYST-A when administered with the toxin into the isolated ileal loop preparation. Pancreatic enzymes and protein-binding were examined as 2 possible causes for the low oral toxicity of MCYST-A. Pancreatic enzymes added to MCYST-A in the ileum or incubated with the toxin before introduction into ileum lowered its toxicity. Addition of bovine serum albumin to MCYST-A placed in the isolated ileal loop caused similar reduction in toxicity suggesting that nonspecific protein-binding may play a role in the tolerance of laboratory animals to orally administered toxin. Keywords: Phytotoxins, Microsistis, Microsistis aeruginosa.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1987
- Accession Number
- ADA203948
Entities
People
- Andrew M. Dahlem
- Val R. Beasley
- Wanda M. Haschek-hock
- Wayne W. Carmichael
- William O. Cook
Organizations
- University of Illinois Urbana–Champaign