Regulation of Neuronal Muscarinic Acetylcholine Receptors

Abstract

The original goal of this proposal was to determine the effects of electrical depolarization on muscarinic acetylcholine receptors (mAChR) in the cultured neuroblastoma cell line, N1E-115. Previous results in a variety of systems suggested that electrical activity might regulate the number of mAChR in neuronal cells; this research was designed to examine this phenomenon in a defined cell line amenable to growth in cell culture, so that the molecular and cellular mechanisms responsible for these changes could be determined. During the course of these studies, experiments were also undertaken to examine the effects of depolarization on GTP-binding regulatory proteins (G-proteins) and the effects of pertussis toxin, lithium, and phorbol ester treatments on muscarinic receptors in these cells. The gene encoding a neuronal form of the MAChR was isolated, and the function of neuronal mAChR expressed from cloned genes in non-neuronal cells was examined.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1989
Accession Number
ADA204296

Entities

People

  • Neil M. Nathanson

Organizations

  • University of Washington

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Anatomy
  • Biological Sciences
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Coding
  • Culture Techniques
  • Depolarization
  • Kinases
  • Military Research
  • Neuroblastoma
  • Notation
  • Regulations
  • Security
  • Steady State

Fields of Study

  • Biology

Readers

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