Molecular Basis of Pathogenicity in Enteric Bacteria

Abstract

A new quantitative assay for the determination of the E. coli heat- stable enterotoxin has been developed which is about 10-fold more sensitive than existing methods. A strategy for the development of subunit vaccines has been tested using specific peptides of the binding region of cholera toxin. Although there have been numerous reports of the relationship between E. coli LT toxin (and cholera toxin) and a putative enterotoxin of Salmonella typhimurium, we have been unable to demonstrate any DNA sequence homology between known toxin sequences of E. coli LT, ST or cholera toxin with the nucleic acid of Salmonella. Keywords: Laboratory animals; Bacteria; Genetics; Antimicrobials; Drug resistance; Biotechnology; Diagnosis; E. coli; Drugs; Enterobacteriaceae; Genetic engineering; Plasmids; Salmonella typhimurium; Enterotoxins; Vaccines.

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Document Details

Document Type
Technical Report
Publication Date
Mar 31, 1985
Accession Number
ADA204307

Entities

People

  • Stanley Falkow

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Animals
  • Anti-Infective Agents
  • Bacteria
  • Carrier Proteins
  • Cells
  • Chemistry
  • Gammaproteobacteria
  • Genetic Engineering
  • Genetic Structures
  • Genetics
  • Laboratory Animals
  • Microbiology
  • Microorganisms
  • Molecular Biology
  • Vaccines

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology