Development of an Immunoassay for Bacterial Endotoxins

Abstract

The goal of this study was to develop an assay for lipopolysaccharides (LPS) which would be as broadly sensitive to LPS from as diverse a group of gram negative bacteria as possible. Sixty different LPS reactive monoclonal antibodies were generated from Salmonella minnesota Re595 bacteria and germ-free mice. Cross reactive antibodies were screened for using a panel of structurally different LPS types. The data obtained resulted in a scoring system which allowed selection of several monoclonal antibodies as most likely to be useful in the LPS assay. During the antibody study a lipopolysaccharide binding protein (LBP) from rabbit acute phase serum was discovered and found to have broader LPS binding properties than the monoclonal antibodies. These results offer several strategies for development of a broadly sensitive LPS detection system.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1986
Accession Number
ADA204316

Entities

People

  • Peter S. Tobias
  • Richard J. Ulevitch

Organizations

  • Scripps Research

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Albumins
  • Antibodies
  • Bacteria
  • Carrier Proteins
  • Cell Line
  • Cells
  • Detection
  • Immunoassay
  • Lipids
  • Lipopolysaccharides
  • Membranes
  • Proteins
  • Reactivities
  • Security
  • Statistical Sampling

Fields of Study

  • Biology

Readers

  • Allergy and Immunology.
  • Immunology
  • Molecular and Cellular Biochemistry