Structure and Function of Cytochrome P-450 Genes
Abstract
The overall goal is to understand the regulated biosynthesis of rabbit liver cytochrome P450. To achieve this goal, we are studying the structure of the genes for these enzymes, the mechanisms by which phenobarbital induces the activity of the enzymes and the nature of the mRNA's. The cytochrome P450IIC (previously designated P-450PBc) subfamily contains several closely related members. Prior to this grant, cDNA's for three of these members had been isolated and sequenced and a portion of one of the genes, cytochrome P450IIC2, had been characterized. During this granting period, a fourth cDNA for cytochrome P450IIC4 was identified and characterized, genomic fragments, including the 5' flanking regions, were characterized for three other genes, the introduction of cytochrome P450IIC4 by phenobarbital was demonstrated, induction by phenobarbital of cytochrome P450IIC1/2/4 was shown to be largely accounted for by an increase in transcription rates, the probable correspondence of P450IIC2 to kidney cytochrome P450K was established, and the number of closely related genes in the rabbit P450 subfamily was estimated at about 10 on the basis of the size and number of bands in a Southern analysis. Attempts to obtain expression of hybrid genes containing P450IIC 5' flanking regions and a reporter gene in several cell lines were unsuccessful.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 02, 1988
- Accession Number
- ADA204405
Entities
People
- Byron Kemper
Organizations
- University of Illinois Urbana–Champaign