The Key Involvement of Poly (ADP-Ribsylation) in Defense Against Toxic Agents: Molecular Biology Studies
Abstract
Poly (ADP-Rib) polymerase requires DNA for activity. It is significant that the catalytic activity of this enzyme is directly coordinated to the number of DNA strand breaks in DNA, both in vitro as well as in vivo. The poly (ADP-Ribosylation) modification of chromatin-associated proteins functions during various biological reactions involving DNA repair and replication). We have studied how poly (ADP-ribosylation) helps protect cells from toxic agents which interact with DNA. specifically, we have been the first group to have reported the cloning of the cDNA and gene for this enzyme. Our aim during the last year was mainly concerned with how to manipulate the hyperexpression of the cloned gene for this enzyme first, to make cells more resistant to toxic agents, and second, to begin to understand the underlying mechanisms by which ADP-Ribosylation alters chromatin around DNA strand breaks to assist cell recovery from such damage.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 10, 1989
- Accession Number
- ADA204443
Entities
People
- Mark Smulson
Organizations
- Georgetown University