Immunogenic Characterization of the Dengue Virus Specified Nonstructural Glycoprotein GP48 (NV3, Soluble Complement Fixing Antigen)
Abstract
Efforts have been concerned with mechanisms of the protective immune response to the flavivirus nonstructural protein NS1 and identification of protective NS1 domains for incorporation in possible future subunit flavivirus vaccines. We have reported that immunization with yellow fever (YF) NS1 protects mice and monkeys against lethal YF infection and that mice immunized with dengue (DEN)-2 NS1 are similarly protected against the flavivirus. These observations have since been confirmed and extended - immunization of mice with a YF NS1 recombinant fusion protein conferred partial protection against the virus (Cane & Gould, 1988) and most recently C.J. Lai and coworkers at NIH have demonstrated solid protection after lethal DEN 4 challenge in mice actively immunized with recombinant DEN 4 NS1 alone or in the form of a polyprotein, produced in a baculovirus vector. Cumulative data from assays which measure the capacity of anti-NS1 monoclonal antibodies (Mab) to sensitive cells to complement-mediated lysis suggests a correlation between such activity and Mab protective capacity. Immune recognition of viral antigen on the surface of infected cells may provide a mechanism of host defense and recovery in flavivirus infection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 09, 1988
- Accession Number
- ADA204600
Entities
People
- Jacob J. Schlesinger
Organizations
- Rochester General Hospital