High-Performance Liquid Chromatography (HPLC) Measurements of Phytoplankton Pigment Distributions of Ocean Waters

Abstract

Unit the application of high-performance liquid chromatography (HPLC) to phytoplankton pigment analysis, it was difficult to quantitatively measure these pigment compounds. HPLC, the state-of-the-art method for the separation and quantification of photosynthetic pigments, is detailed in this report. HPLC reduces interferences caused by overlapping absorption and fluorescence bands of various pigments. HPLC-derived pigment distributions were measured in the upper 200 meters during two NOSC-sponsored cruises on the USNS LYNCH on 1-22 August 1986 and 29 July -18 August 1987 in the Norwegian, Barents, and Greenland Seas. Carotenoids, Hydrocasts, Fluorescence, Particulates, Pigments, Sea surface temperature, Undulating oceanographic recorder (UOR), Chlorophyll, Coccolithophorids.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1988
Accession Number
ADA204760

Entities

People

  • C. C. Trees

Organizations

  • Scripps Institution of Oceanography

Tags

Communities of Interest

  • Space

DTIC Thesaurus Topics

  • Absorption
  • Abstracts
  • Chromatography
  • Classification
  • Detectors
  • Fluorescence
  • Greenland Sea
  • Instrumentation
  • Liquid Chromatography
  • Measurement
  • Oceanography
  • Oceans
  • Optical Properties
  • Phytoplankton
  • Plankton
  • Sea Surface Temperature
  • Surface Temperature

Readers

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  • Oceanography.
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