The Primary Sequence of Acetylcholinesterase and Selective Antibodies for the Detection of Organophosphate Toxicity
Abstract
The proposed work has been directed to determination of the structure of acetylcholinesterase. During the project period we have determined the primary structure of the Torpedo enzyme through amino acid sequencing and the isolation and sequencing of a cDNA clone encoding for the 11s form of the enzyme. The recombinant DNA studies involving the isolation of cDNA clones encoding acetylcholinesterase were part of studies supported by an NIH grant. However, they relied on amino acid sequence information for preparation of oligonucleotides for screening and ultimately yielded complementary information on primary structure. Peptides corresponding to the active center of the enzyme and a C-terminal region have been synthesized and antibodies are being raised for the purpose of detecting the phosphorylated enzyme and delineating functional regions of the molecule. Finally, the disulfide bonding pattern has been determined for the 11s form of the enzyme.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1987
- Accession Number
- ADA204831
Entities
People
- Palmer W. Taylor
Organizations
- University of California, San Diego