Novel Approaches to the Study of Synaptic Function, xa
Abstract
The original goal of the proposed experiments was to explore the possibility that mRNA-injected Xenopus oocytes could be used to reconstitute the transmitter release process. The hypothesis was that it might be possible to detect physiologically relevant acetylcholine release from Xenopus oocytes injected first with mRNA from cholinergic neurons and then with synaptic vesicles. For a number of technical reasons that have been discussed in prior reports, this approach has not been successful. In lieu of this effort, our primary focus has shifted toward attempts to clone the cDNA for two presynaptic proteins (the N-type calcium channel and the high affinity choline transporter) and one post-synaptic protein (a glutamate receptor). The status of these efforts is outlined in this report.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 16, 1989
- Accession Number
- ADA204842
Entities
People
- Cameron B. Gundersen
Organizations
- University of California, Los Angeles