Protein-Modified Electrochemically Active Biomaterial Surface

Abstract

This research was initiated with a novel approach to electrode immobilization of an enzyme, glucose oxidase, by electropolymerization of pyrrole in the presence of the enzyme. The enzyme was entrapped by the polypyrrole film growing on the electrode surface. Since polypyrrole is an electronically conducting organic polymer, the effect of this immobilization was to enfold the enzyme in a conducting electrode. In addition, the products of the enzyme reaction with glucose were produced in the immediate proximity of this conducting material. The electropolymerized enzyme is active and serves as the basis for a glucose electrode system which remained active for several days. The conditions for electro-polimerization and the characteristics of the immobilized enzyme system were reported in the open literature.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1988
Accession Number
ADA205154

Entities

People

  • Mirtha Umana

Organizations

  • Purdue University

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Antibodies
  • Biomaterials
  • Biosensors
  • Carboxylic Acids
  • Chemical Synthesis
  • Chemistry
  • Conductive Polymers
  • Detection
  • Detectors
  • Electron Transfer
  • Electrons
  • Immobilized Enzymes
  • Immunoassay
  • Materials
  • Military Research
  • Polymers
  • Proteins

Fields of Study

  • Chemistry

Readers

  • Electrochemical Surface Science
  • Molecular and Cellular Biochemistry

Technology Areas

  • Microelectronics