Polysome Immunoselection Combined with cDNA Cloning to Obtain Specific Genes from Chlamydomonas reinhardtii

Abstract

The research objective is to develop the capability to obtain full- copy structural genes from C. reinhardtii by immunopurification of specific MRNAs from polysomes combined with efficient cDNA synthesis and cloning. In this research project, techniques for immunoselection of polysomes and cloning of full-length cDNA from the single-celled alga, Chlamydomonas reinhardtii CW15, have been developed. A C. reinhardtii cDNA library has been constructed and initial characterization has shown that the cloned cDNA is the size of full length mRNAs and the cloned cDNA hybridizes to C. reinhardtii cDNA derived from purified polysomal poly(A+) RNA. With these capabilities, immunopurification of specific mRNAs can be combined with efficient cDNA cloning to obtain specific full-copy structural genes.

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Document Details

Document Type
Technical Report
Publication Date
Feb 05, 1989
Accession Number
ADA205211

Entities

People

  • Paul S. Tabor

Organizations

  • Indiana State University

Tags

Communities of Interest

  • Materials and Manufacturing Processes
  • Weapons Technologies

DTIC Thesaurus Topics

  • Antibodies
  • Biochemistry
  • Biological Sciences
  • Biology
  • Biotechnology
  • Cells
  • Chemistry
  • Classification
  • Elements
  • Hybridization
  • Magnesium
  • Molecular Biology
  • Nitrogen
  • Phosphodiesterases
  • Potassium
  • Potassium Chloride
  • Potassium Compounds

Fields of Study

  • Biology

Readers

  • Molecular Genetics