Immunological Approach to the Identification and Development of Vaccines to Various Toxins
Abstract
Monoclonal antibodies specific for the sodium channel blockers saxitoxin (STX) and tetrodotoxin (TDT) have been generated and characterized as to their abilities to protect against toxin-induced in vitro toxicity and to induce anti-idiotypic immune responses. In a continued effort to enhance the binding affinities of the anti-toxin monoclonal antibodies, two novel approaches have been undertaken. The first one involves the design of a new toxin conjugate for use in immunization of BALB/c mice. The second approach involves the in vitro stimulation with free STX or TDT of mouse spleen cells hyper-immunized in vivo. Monoclonal anti-TDT and anti-STX antibodies have been generated that are protective against TDT and STX toxicity, respectively, on nerve functions. Rabbit anti-idiotypic antibodies specific for TD2C5 anti-TDT have been generated, purified and used in immunization of mice in order to test the potential use of anti-idiotype as a vaccine in inducing a systemic anti-TDT immune response. Keywords: Monoclonal antibodies, In situ protection, Anti- idiotypic vaccines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 07, 1989
- Accession Number
- ADA205366
Entities
People
- Deborah Armstrong
- Ronald Kennedy
- Tran C. Chanh
Organizations
- Texas Biomedical Research Institute