Hepatic Subcellular Distribution of (3H)T-2 Toxin
Abstract
The subcellular distribution of T-2 mycotoxin and its metabolites was studied in isolated rat livers perfused with (3 tritium)T-2 toxin. After a 120- min perfusion, the distribution of radiolabel was to bile (53%), perfusate (38), and liver (7%). Livers were fractionated into mitochondria, endoplasmic reticulum (smooth and rough), plasma membrane, and nuclei. Plasma membrane fractions contained 38% of the radiolabel within 5 min, decreasing to < 1% at the end of the 120-min perfusion. Smooth endoplasmic reticulum contained 27% of the radiolabel by 5 min and increased to 43% over the 120-min perfusion. The mitochondrial fraction contained 3% of the radiolabel by 30 min and increased to 10% after 120-min perfusion. Label in the nuclear fraction remained constant at 7% from 30 to 120 min. By 15 min, only the parent toxin was detected in the mitochondrial fraction. In the other fractions, radiolabel was associated with HT-2, 4-deacetylneosolaniol, T-2 tetraol, and glucuronide conjugates. Glucuronide conjugates accounted for radiolabel eliminated via the bile. The time course for distribution of radiolabel in liver suggested an immediate association of (3H)T-2 with plasma membranes and a subsequent association of toxin and metabolites with endoplasmic reticulum, mitochondria, and nuclei, the known sites of action of this toxin. Keywords: Phytotoxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 10, 1989
- Accession Number
- ADA205418
Entities
People
- Judith G. Pace
- Michael. R. Watts
Organizations
- United States Army Medical Research Institute of Infectious Diseases