Effect of Anti-Hepatotoxic Agents Against Microcystin-LR Toxicity in Cultured Rat Hepatocytes
Abstract
Primary cultures of adult rat hepatocytes were used to investigate the effects of two putative therapeutic agents, dithioerythritol and silymarin on microcystin-LR-induced hepatotoxicity. Cell injury was assessed by (1) the extent of cellular 14C adenine nucleotides and lactate dehydrogenase (LDH) release into the media and (2) the extent of hepatocyte detachment from monolayers. Microcystin LR (1 micromoles) induced a significant release of both 14C nucleotides and LDH from hepatocytes as well as significant detachment of cells from monolayers. Although both dithioerythritol and silymarin reduced the amount of marker release and cell detachment from microcystin-LR-treated wells, silymarin provided significantly greater protection than dithioerythritol at one tenth the concentration. Furthermore, silymarin and dithioerythritol treatment prevented morphological deformations and detachment of cells. Keywords: Antitoxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 10, 1989
- Accession Number
- ADA205493
Entities
People
- Kay A. Mereish
- Rikki Solow
Organizations
- United States Army Medical Research Institute of Infectious Diseases