Patch-Clamp Studies in Human Macrophages: Single-Channel and Whole-Cell Characterization of Two K+ Conductances
Abstract
Human peripheral blood monocytes cultured for varying periods of time were studied using whole-cell and single channel patch-clamp recording techniques. Whole-cell recordings revealed both an outward potassium current activating at potentials > 20 mV and an inwardly rectifying K current present at potentials negative to -60 mV. Tail currents elicited by voltage steps that activated outward current reversed near Ek, indicating that the outward current was the single-channel current. Excised and cell-attached single-channel data showed that calcium-activated K channels were Absent in freshly isolated monocytes but were present in > 85% of patches from macrophages cultured for >7 days. Only whole-cell inward currents. The inward current was blocked by external barium and increased when K10 increased. Inward-rectifying single- channel currents with a conductance of 28 pS were present in cells exhibiting inward whole-cell currents. These single-channel currents are similar to those described in detail in J774. 1 cells. Keywords: Ion channels, Ionic current, Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA205801
Entities
People
- Elaine K. Gallin
- Leslie C. Mckinney
Organizations
- Armed Forces Radiobiology Research Institute