Comparison of In vivo Effects of Human Recombinant IL 1 and Human Recombinant IL 6 in Mice
Abstract
Interleukin 1 and Interleukin 6 share a number of biological activities, including induction of fever, neutrophilia and acute phase response, and IL 1 induces IL 6 production by fibroblasts and macrophages. Therefore, it was proposed that IL 6 mediates many of the activities of IL 1. To test this hypothesis in vivo, we assessed induction of IL 6 following IL 1a administration to mice and tested IL 6 for radioprotection and induction of early (CSF) and late (fibrinogen and SAA) acute phase reactants. Unlike IL 1 which is radioprotective when administered in doses above 100 ng/mouse, doses of 10-1000 ng/mouse of human recombinant IL 6 did not result in increased survival of mice following lethal irradiation. Such treatment given 20 hrs before LD 50/30 doses of radiation resulted in reduced survival of mice. However, IL 6 augmented the radioprotective effect of IL 1. IL 1 in doses above 10 ng/mouse induced within 2 to 6 hrs a dose dependent increase in CSF in circulation, but IL 6 did not induce detectable levels of CSF at 2, 6 and 20 hrs after administration. Administration of IL 6 to mice produced a dose dependent increase in circulating fibrinogen and SAA. Similarly, administration of IL 1 resulted in much greater increases in levels of fibrinogen and SAA. Therefore, IL 1 is a more effective inducer of fibrinogen and SAA in mice than is IL 6. Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1988
- Accession Number
- ADA205813
Entities
People
- Gordon G. Wong
- Jean D. Sipe
- Richard P. Nordan
- Ruth Neta
- Stefanie N. Vogel
Organizations
- Armed Forces Radiobiology Research Institute