Molecular Specificity of Adsorption of Biofilm Macromolecules and Microbial Biofouling on Artificial Surfaces in the Sea
Abstract
An ELIZA method using immune chicken IgG coated plates does not appear to be specific for microbial adhesion enhancing macromolecules (MAEM). Hyperimmune mouse IgG made against MAEM( but not normal mouse IgG) reacts significantly with coated plates in the presence or absence of MAEM antigens. Assays yield titers of approximately 100,000 for the hyperimmune mouse sera over that of normal control. Hyperimmune mouse sera consistently showed this property despite modifications in the assay method. The very high titer of hyperimmune mouse serum suggests that immunization has produced an antibody(ies) against MAEM antigens, however, the assay could not discriminate specific from nonspecific reactions. Efforts will continue to focus on the problem, by examining the effects of D-galactose and other simple sugars on this binding. Elevated salt concentrations will be examined as a means of minimizing non-antigen mediated reactions. Keywords: Hybridoma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 06, 1989
- Accession Number
- ADA205874
Entities
People
- Thomas R. Tosteson
- Yasuhino Yamamura
Organizations
- University of Puerto Rico at Mayaguez