The Effects of Recombinant Interleukin-1 and Interleukin-2 on Human Keratinocytes Running Head: Interleukins-1 and 2 Effect on Keratinocytes
Abstract
The effects of recombinant interleukin-1 alpha and beta, as well as recombinant interleukin-2, on human keratinocyte proliferation were studied in serum-containing as well as defined media. Both interleukin-1 preparations did not stimulate keratinocyte growth; interleukin-2 also did not stimulate keratinocyte growth. To determine whether interleukin-1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin-1 beta binds to keratinocytes with a Kd of 6.2 nM and 2,500 receptors per cell. To determine the effects of interleukin-1 beta on protein synthesis, the molecular patterns of radio-labeled cell extracts of interleukin-1 beta-treated and non-treated keratinocytes were compared using 2-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1987
- Accession Number
- ADA206079
Entities
People
- Anastasia B. Cua
- Gregory J. Wastek
- Jonathan N. Mansbridge
- Vera B. Morhenn
Organizations
- Stanford University Medical Center