Molecular Cloning of Human Gene(s) Directing the Synthesis of Nervous System Cholinesterases

Abstract

Cholinesterases (ChEs) are highly polymorphic serine hydrolases involved in the termination of neurotransmission in cholinergic synapses and neuromuscular junctions. Both the levels and the molecular forms of ChEs were shown to be modulated during development, denervation and regeneration processes in various species. In order to examine the molecular mechanisms underlying these phenomena in humans, we have used oligodeoxynucleotide probes to isolate full-length cDNA clones coding for human ChE. These clones are currently employed in our laboratory to study the biogenesis of human ChE at various levels of gene expression. Keywords: Cholinesterase; Chromosomal mapping; cDNA hybridization; Genomic clones; Xenopus oocytes microinjection.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1987
Accession Number
ADA206405

Entities

People

  • Soreq Hermona

Organizations

  • Weizmann Institute of Science

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  • Biomedical

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Fields of Study

  • Biology

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  • Molecular Genetics
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