Gamma Interferon Reduces the Synthesis of Fibronectin by Human Keratinocytes
Abstract
Recombinant gamma interferon has a variety of effects on human keratinocytes including the induction of synthesis and expression of HLA-DR antigen as well as growth inhibition. In order to ascertain whether rIFN-y affects the keratinocytes capacity to interact with other skin cells and potentially alter the composition of skin proteins, we tested the effect of RIFN-y on the secretion of proteins by keratinocytes in vitro. Keratinocytes grown in serum free medium were treated with increasing concentrations of rIFN- y(3 U/ml-1000 U/Ml). The cells were radiolabeled with 35-S-methionine and the supernatants were harvested, excess 35-S-methionine removed, and the proteins analyzed by polyacrylamide gel electrophoresis. The relative synthesis of several proteins was altered by rIFN-y treatment. In particular rIFN-y decreased the synthesis of two proteins, one with a molecular weight of approximately 250 kD and the other 180 kD, adn increased the synthesis of an approximately 100 kD protein in a dose dependent manner. Immunoprecipitation with polyclonal anti- fibronectin antibody showed that the 250 kD protein is the fibronectin monomer. No smaller fragments of fibronectin were immunoprecipitated, suggesting that the reduction in extracellular fibronectin following treatment by rIFN-y was not a direct result of proteolytic enzyme digestion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 06, 1988
- Accession Number
- ADA206645
Entities
People
- Jonathan N. Mansbridge
- Shelia T. Huang
- Vera B. Morhenn
Organizations
- Stanford University