Gamma Interferon-Induced Expression of Class II MHC Antigens by Human Keratinocytes: Effects of Conditions of Culture
Abstract
Normal human keratinocytes grown in MCDB 153 plus bovine pituitary extract and treated with recombinant gamma interferon (rIFN gamma) express HLA- DR, DP and DQ antigens. The expression of these class II Major Histocompatibility Complex antigens is time dependent: DR and DP appear before DQ. The delay in the HLA-DQ expression is not due to the effects of trypsinization of cultures prior to analysis. Increasing the calcium ion concentration from 0.1 to 1.8 mM does not alter the expression of these antigens. Keratinocytes grown without serum proteins or bovine pituitary extract exhibited markedly delayed expression of DR. By contrast, keratinocytes grown in Dulbecco's modified Eagle's medium (DMEM) plus 10% fetal calf serum express DR and DP but only very small amounts of DQ after treatment with rIFN gamma. Expression of HLA-DR occurs at doses of rIFN gamma that are too low to cause growth inhibition. The cells of the squamous cell line SCL-1, whether grown in MCDB 153 plus bovine pituitary extract or DMEM plus 10% fetal calf serum, express HLA-DQ and DP on only small numbers of cells after treatment with the lymphokine. Thus, the conditions of culture, possibly the presence of a serum factor(s), influence the expression of class II antigens in normal keratinocytes. Furthermore, rIFN gamma does not induce DP and DQ antigens readily in transformed squamous cells cultured in either serum-containing or serum-free medium.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1987
- Accession Number
- ADA206647
Entities
People
- Gary S. Wood
- Vera B. Morhenn
Organizations
- Stanford University Medical Center