Description and Manipulation of Membrane Lipid Alterations Associated with Synaptic Functions in Isolated Cerebellar Glomeruli
Abstract
Isolated cerebellar and Hippocampal mossy fiber synaptosomes were used to assess the relationships between membrane lipid metabolism and the evoked release of excitatory amino acid neurotransmitters. Mossy fiber terminals were radio-labeled with arachidonic acid and the effects of membrane depolarization and calcium influx on the labeling of the component lipid pools were determined. It was observed that depolarization and Ca2+ influx stimulated the accumulation of unesterified arachidonate. This effect was correlated with increased production of prostaglandins, and lipoxygenase inhibitors. The depolarization and arachidonate-induced transmitter release was mimicked by the addition of PGE2a and blocked by the cyclooxygenase inhibitor ibuprofen. The correlation of arachidonic acid metabolism with prostaglandin synthesis, ATPase inhibition and Ca2+ mobilization, suggests they may all play a role in the mechanisms of neurotransmitter release.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 17, 1989
- Accession Number
- ADA206732
Entities
People
- Robert V. Dorman
Organizations
- Kent State University