Molecular Biology of Archaebacteria
Abstract
Transcripts derived from the 5.2 Kbp fragment of Halobacterium cutirbrum genomic DNA that encodes the Llle, Lle, L10e and L12e ribosomal protein genes have been characterized. The leader region of the Lle, L10e, 112e tricistronic transcript contains a region that exhibits sequence and structural similarity to the Lle binding site in 23S rRNA. This leader sequence is presumably used to autogenously regulate mRNA translation. The gene encoding the H. cutirubrum superoxide dismutase has been cloned and sequences and its transcript has been characterized. Transcription of the gene is inducible by oxygen radicals. The promoter regions from the two rRNA operons of H. Marismortui have been sequenced and the promoters have been defined by nuclease protection. The 5' flanking region of these two operons differ substantially with regard to i) the number of promoters, ii) the processing signals, and iii) nucleotide sequence of the first 130 nucleotide within the 16S gene. Finally, a family of five genes encoding multi-forms of the eucaryotic L12e protein and a single L10e protein have been cloned, transcript mapped, and compared to the corresponding eubacterial and archaebacterial proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 31, 1989
- Accession Number
- ADA206740
Entities
People
- Patrick P. Dennis
Organizations
- University of British Columbia