Dengue 4 Vaccine Development
Abstract
A live attenuated Dengue 4 virus candidate vaccine was prepared by serial passage of wild virus isolates in primary dog kidney cells (PDK) at 32 C. In addition, a candidate challenge wild type virus was prepared from the wild type parent by limited passage at 37 C in primary African green monkey kidney cells (PGMK). These viruses were finally passaged in fetal rhesus lung (FRhL) cells for preparation of Master Seed and Production Seed stocks. An additional passage in FRhL cells to produce a candidate vaccine was done at WRAIR from the Production Seeds. All virus passages and lots of cell cultures were routinely tested for sterility according to a standard protocol. The Production Seeds and Final Product (Candidate vaccine) were safety tested and determined to be free of contamination with bacteria (including mycoplasma), fungi, and adventitious agents according to standard protocols. Biological marker studies were done on Production Seeds. Keywords: Virus, Arbovirus, Dengue, Dengue-4, Vaccines, Attenuation, Immunity, RAI.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1987
- Accession Number
- ADA206953
Entities
People
- Nyven J. Marchette
Organizations
- University of Hawaiʻi System