Acute Intravenous Toxicity Study of Hypertonic Saline/Dextran 70 (Trade Name) and Constituents in ICR Mice
Abstract
The acute toxicity following intravenous administration of a proposed new resuscitation fluid, hypertonic saline/Dextran 70 (HSD), was evaluated in male and female ICR mice. Animals were administered a single intravenous dose of HSD, 28 m1/kg over a 5-minute period, in an attempt to define its maximum tolerated dose. Equal volumes of each HSD component, 7.5% hypertonic saline (HS) and 6% Dextran 70 (D70) in normal saline, and Ringer's lactate (RL) were also evaluated. Major signs observed in all groups were hunched posture, rough coat, hyperactivity, inactivity, and increased rate and/or depth of respiration. Body weights were unaffected by the dosing and no gross or microscopic lesions could be attributed to HSD or its constituents. These data suggest that the maximum tolerated dose of HSD following acute intravenous administration is less than 28 ml/kg, and that the toxicity associated with HSD is consistent with the administration of large volumes of hypertonic saline. Since the proposed therapeutic dose of HSD is only 4 ml/kg, these findings indicate that there will be minimal adverse effects associated with the therapeutic administration of HSD.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1988
- Accession Number
- ADA207247
Entities
People
- Denzil F. Frost
- Don W. Korte Jr.
- G. D. Young
- Gary M. Zaucha
Organizations
- Letterman Army Hospital