In vitro and In vivo Studies for Development of a Leishmaniasis Vaccine

Abstract

The goal of this contract is to identify, characterize , and test antigens of Leishmania that could serve as candidate vaccines against cutaneous leishmaniasis. Monoclonal antibodies were raised against promastigotes and tested for their reactivity with leishmanial antigens using immunoprecipitation and Western blot analysis. A radioimmunoassay was developed to detect the presence of leishmanial antigens on the surface of infected P388D1 cells, and was employed to screen monoclonal antibodies for their ability to detect such antigens. Keywords: Macrophages; Antileishmanial drugs.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Mar 03, 1988
Accession Number
ADA207362

Entities

People

  • David J. Wyler

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Antibodies
  • Biomedical And Dental Materials
  • Blood
  • Cells
  • Contracts
  • Health Services
  • Immune Serums
  • Infection
  • Leishmania
  • Leishmaniasis
  • Parasites
  • Polymeric Films
  • Proteins
  • Reactivities
  • Vaccines

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech