Chemical Blistering: Cellular and Macromolecular Components

Abstract

The overall objective of this investigation is to elucidate the molecular mechanisms by which mustard exerts its chemical blistering action when applied topically to human skin. The study involves the use of keratinocyte tissue cultures in order to focus on the direct interactions between the mustard and the cellular targets. The technical objectives of the project are to develop appropriate culture systems for use in the investigation of subcellular and macromolecular manifestations of toxicity, to establish the credibility of these systems for investigating the molecular mechanisms of these effects, and to determine whether these systems can be used-to develop procedures by which toxic responses can be neutralized. Mustard agents, Keratinocytes, Tissue culture, Alkylation, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA repair, Epidermal proliferation, Epidermal differentiation collagen.

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Document Details

Document Type
Technical Report
Publication Date
Dec 15, 1985
Accession Number
ADA208408

Entities

People

  • Abhishek N. Kulkarni
  • I. A. Bernstein
  • M. J. Brabec
  • R. C. Conolly
  • R. H. Gray

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Biochemistry
  • Cells
  • Chemistry
  • Chlorides
  • Culture Techniques
  • Epidermis
  • Epithelial Cells
  • Fish
  • Growth Factors
  • Health Services
  • Laboratory Animals
  • Materials
  • Metabolism
  • Mitochondria
  • New York
  • Subcellular Fractions
  • Synthetic Membranes

Readers

  • Analytical Chemistry
  • Cellular and Molecular Pathways of Apoptosis.
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology