Effects of Pressure on Membrane-Associated Receptors and Effector Elements
Abstract
The effects of hydrostatic pressure (1 atm to 408 atm) on the A1 adenosine receptor-Gi protein-adenylate cyclase complex were studied in membrane preparations of two congeneric marine teleost fishes, Sebastolobus alascanus and S. altivelis. S. alascanus is common from 180-440 m; S. altivelis from 550-1300 m. Assay conditions for the binding of A1 adenosine receptor agonists at 5 C and atmospheric pressure were optimized and the reaction conditions for the assay of adenylate cyclase were determined. The effects of hydrostatic pressure on basal adenylate cyclase activity and the response of adenylate cyclase to modulation by A1 receptor occupancy were determined. Basal adenylate cyclase activity was inhibited 11 to 25% by 136 atm pressure and 29 to 41% by 408 atm. Inhibition by the A1 adenosine receptor agonist cyclopentyladenosine (0.1 mM) was affected by pressure increases. (32P)ADP-ribosylation of G protein substrates differed 10 fold between the Sebastolobus congeners and the extent of (32P)ADP-ribosylation was altered by pressure increases. All of the components tested on this receptor-effector system display sensitivity to pressure changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 16, 1989
- Accession Number
- ADA209573
Entities
People
- Joseph F. Siebenaller
- Thomas F. Murray
Organizations
- Oregon State University