Phylogenetic Analysis of Marine Picoplankton Using rRNA Sequences

Abstract

The purpose of the program is to analyze phylogenetically the dominant constituents of marine picoplankton in selected Atlantic and Pacific Oceans oligotrophic sites using 16S ribosomal RNA gene cloning and sequencing procedures. Additional goals are the development of rRNA-based hybridization probes for the phylogenetic analysis of single cells and the continued accumulation of 16S rRNA reference sequences. Recent efforts have resulted in the establishment of a recombinant DNA library of Sargasso Sea and northern Pacific picoplankton, the development of fluorescence-based, phylogenetic group- specific hybridization probes, and a continuing phylogenetic survey of cultivated cyanobacteria. Keywords: Phylogeny; Taxonomy; Marine picoplankton; 16S rRNA phylogeny; Recombinant DNA; Nucleic acid hybridization; Nucleotide sequence analysis.

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Document Details

Document Type
Technical Report
Publication Date
Jun 20, 1989
Accession Number
ADA209595

Entities

People

  • Norman R. Pace

Organizations

  • Indiana University Bloomington

Tags

DTIC Thesaurus Topics

  • Availability
  • Biology
  • Cells
  • Classification
  • Contracts
  • Cyanobacteria
  • Databases
  • Deoxyribonucleic Acids
  • Fluorescence
  • Genetic Structures
  • High Temperature
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Recombinant Dna
  • Sargasso Sea
  • Security
  • Sequence Analysis

Fields of Study

  • Biology
  • Environmental science

Readers

  • Molecular Genetics
  • Oceanography.

Technology Areas

  • Biotechnology