Heterosynaptic Modulation of Long-Term Potentiation at Mossy Fiber Synapses in Hippocampus
Abstract
The overall goal of this research project is to investigate the cellular mechanisms associated with the heterosynaptic modulation of long-term synaptic potentiation (LTP) at mossy fiber synapses in hippocampus. It was previously shown that norepinephrine, through Beta-adrenoceptors, enhances the magnitude, duration, and probability of induction of mossy fiber LTP, while acetylcholine, through muscarinic receptors, depresses the magnitude and probability of induction of mossy fiber LTP. The goal for the first year of this research project was to test several specific hypotheses for the cholinergic and entry through voltage-gated calcium channels for the induction of LTP. We have also been investigating the properties of voltage-ated calcium channels in hippocampal CA3 neurons and the modulation of these calcium channels by noradrenergic and cholinergic agonists. These studies have used acutely exposed hippocampal neurons and a new preparation of isolated mossy fiber presynaptic terminals. In a collaborative project with Dr. David Terrian at the USAFSAM, San Antonio, the mechanisms of neurotransmitter release from a homogeneous fraction of mossy fiber synaptosomes have been investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 16, 1989
- Accession Number
- ADA209835
Entities
People
- Daniel P Johnson
Organizations
- Baylor College of Medicine