Protein Dynamics Studied with Resonantly Enhanced Quasi-Elastic Light

Abstract

In order to investigate and understand the dynamics of protein fluctuations, specifically the interaction between dynamical structure and function, we have started a program to use resonantly enhanced light scattering to probe the fluctuation spectrum. Here we report on a new technique, the use of a quartz microbalance to confirm the existence of motion within myoglobin at 5 MHz. We report on modelling the temperature rise for an absorbing sample with incident laser light. Finally, we give some preliminary results of quasi-elastic light scattering on samples of membrane fragments containing the bacteria Bacteriorhodopsin. Keywords: Raman spectroscopy; Chromophores; Membranes biology; Protein dynamics; Light scattering; Resonance enhancement; Microbalance; Hydration shell; Thermal diffusion; Purple; Membrane.

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Document Details

Document Type
Technical Report
Publication Date
Jul 15, 1989
Accession Number
ADA209971

Entities

People

  • Lou Reinisch

Organizations

  • Uniformed Services University of the Health Sciences

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biochemistry
  • Biological Sciences
  • Biology
  • Biophysics
  • Biotechnology
  • Cell Membrane
  • Chemical Engineering
  • Chemistry
  • Dynamics
  • Engineering
  • Light Scattering
  • Military Research
  • Molecular Biology
  • Physics
  • Scattering
  • Schools
  • Universities

Fields of Study

  • Physics

Readers

  • Molecular and Cellular Biochemistry
  • Optical Physics and Photonics.
  • Thermal Physics or Thermal Science.

Technology Areas

  • Directed Energy