Monocyte-Derived Interleukin 1: Effects on Norepinephrine-Simulated Aortic Contraction and Phosphoinositide Turnover
Abstract
Medium conditioned by silica-stimulated human peripheral blood monocytes express vascular suppressive activity. Rat aortic rings, after incubation in conditioned medium, exhibited comprised contraction to stimulation by norepinephrine (NE). Maximal contraction (300 + or - 51 mg tension/mg tissue) and sensitivity (-5.91 + or - O.23 M (log EC50) were both reduced in comparison to contraction and sensitivity displayed by rings after incubation in control medium. A polyvalent antibody (Ab) against human interleukin 1 (Il-1) neutralized the suppressive activity in conditioned medium. Rings incubated in conditioned medium containing Ab exhibited normal maximal contraction and a partial restoration of sensitivity to NE. In contrast incubation of rings in control medium supplemented with recombinant human Il-1 resulted in a dose- dependent suppression of aortic contraction to NE that was analogous to the defects induced by monocyte-conditioned medium. No significant differences in NE-stimulated phosphoinositide hydrolysis were present between rings incubated in Ab-treated or untreated conditioned or control media. The data suggest that monocyte-derived Il-1 may have a significant influence on vascular contractile function and that the mechanism by which Il-1 induces vascular dysfunction cannot be demonstrated to involve inhibition of NE-stimulated phosphoinositide metabolism. Keywords: Sepsis; Polyvalent antibody; Vascular contraction; Phorbol; Endotoxic shock; Reprints.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1989
- Accession Number
- ADA210036
Entities
People
- John E. Lueders
- Thomas M. Mckenna
- Wolfgang A. Titius
Organizations
- Naval Medical Research Center