Cloning and Characterization of the RecA Gene of Aquaspirillum magnetotacticum

Abstract

The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1988
Accession Number
ADA210231

Entities

People

  • Amy E. Berson
  • Debra V. Hudson
  • Nahid S. Waleh

Organizations

  • SRI International

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Bacteriophages
  • Chemistry
  • Deoxyribonucleic Acids
  • Digestive System Processes
  • Dna Sequence Analysis
  • Escherichia
  • Escherichia Coli
  • Films
  • Gel Electrophoresis
  • Genes
  • Genetic Structures
  • Genetics
  • Genome
  • Recombinant Dna
  • Sequence Analysis

Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology