Possible Mechanism for Denervation Effect on Wound Healing
Abstract
Basic research is being conducted to investigate the role of transferrin, an iron-transport protein required for cell proliferation, in the neural effect on wound healing and tissue regeneration. The system of tissue repair under investigation is the regenerating limb of the axolotl, in which growth is strictly dependent on unknown factors from peripheral nerves. The rationale of the study is to measure and localize transferrin in normal and in denervated limb tissues, obtaining information with which to test the hypothesis that nerves contribute transferrin to cells of the regenerating tissues. Before experiments of this nature can be undertaken, axolotl transferrin and antibodies against this factor must be available so that immunoassays can be developed to measure this protein in nerves, regenerating limbs, and other tissues from axolotls. During the first year of this project, transferrin was purified from axolotls and antisera against it were generated in mice and rabbits. Monoclonal antibodies were also prepared. During the second year (the period of this report), these antibodies have been used to develop an enzyme-linked immunosorbent assay (ELISA) for quantitative measurements of transferrin. Experiments have also begun using these antisera in immunocytochemical studies to localize transferrin in axolotl nervous tissue.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 17, 1989
- Accession Number
- ADA210367
Entities
People
- Anthony L. Mescher
Organizations
- Indiana University