Acute Intravenous Toxicity Study of Hypertonic Saline/Dextran 70 (Trade Name) and Its Constituents in Beagle Dogs
Abstract
The acute toxicity following intravenous administration of a proposed resuscitation fluid, hypertonic saline/Dextran 70 (HSD), was evaluated in male and female beagle dogs. Animals received a single intravenous dose of HSD, 20 ml/kg over a 5-minute period, in an attempt to define maximum tolerated dosage. Observations were made at 1, 2, and 4 hours after administration on the day of dosing and twice daily thereafter. Water consumption was monitored over a 24- hour period weekly during quarantine, daily for the first week of the study, and on Day 14. Clinical signs were observed with increased frequency in the HSD and HS-treated groups and included inactivity, tremors, disorientation (HSD only), salivation, vomiting, and thirst. All observed signs dissipated within 24 hours of dosing, with the exception of an occasional incident of diarrhea and one incident of tremors. No significant changes outside of normal limits were observed in the hematology data. Body weights were unaffected by the dosing and no gross or microscopic lesions could be attributed to HSD or its constituents. Since the proposed therapeutic dose of HSD is only 4 ml/kg, these findings indicate minimal adverse effects should be anticipated with the therapeutic administration of HSD.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1989
- Accession Number
- ADA210664
Entities
People
- Charles B. Clifford
- Denzil F. Frost
- Don W. Korte Jr.
- Gary M. Zaucha
- Stanley T. Omaye
Organizations
- Letterman Army Hospital